Role of RGMc as a Neogenin Ligand in Follicular Development in the Ovary

Kim, Yu Jin and Park, YoungJoon and Park, Yeo Reum and Kim, Young Sang and Lee, Hye Ran and Lee, Sang Jin and Kim, Myung Joo and Kwack, KyuBum and Ko, Jung Jae and Lee, Jae Ho (2021) Role of RGMc as a Neogenin Ligand in Follicular Development in the Ovary. Biomedicines, 9 (3). p. 280. ISSN 2227-9059

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Abstract

There is currently no cure for infertility in women with a poor ovarian response (POR). Neogenin is reported to be abundantly expressed in the ovary; however, its role in mammalian follicular development is unclear and its ligand and signaling pathway remain uncertain. We systematically investigated the role of neogenin and the ligand repulsive guidance molecule c (RGMc) during follicular development. We treated hyperstimulated mouse ovaries with RGMc and analyzed follicular development. Furthermore, we investigated clusters of up/downregulated genes in RGMc-treated ovaries using whole-transcriptome next-generation sequencing (NGS). In addition, we investigated whether expression of up/downregulated factors identified by NGS was also altered in cumulus cells (CCs) of patients with a POR. The number of oocytes was 40% higher in RGMc-treated ovaries than in control ovaries. NGS data indicated that prostaglandin D2 (PGD2) was involved in the RGMc signaling pathway during follicular development. RGMc treatment significantly elevated the PGD2 level in culture medium of CCs obtained from patients with a POR. Our results demonstrate that RGMc as neogenin ligand promotes follicular development in ovaries via the PGD2 signaling pathway. Therefore, it may be possible to use RGMc for ovarian stimulation in patients with a POR.

Item Type: Article
Subjects: STM One > Biological Science
Depositing User: Unnamed user with email support@stmone.org
Date Deposited: 05 Jan 2023 10:20
Last Modified: 20 Apr 2024 08:16
URI: http://publications.openuniversitystm.com/id/eprint/3

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