Brain and Pituitary Transcriptome Analyses Reveal the Differential Regulation of Reproduction-Related LncRNAs and mRNAs in Cynoglossus semilaevis

Dong, Yani and Lyu, Likang and Wen, Haishen and Shi, Bao (2021) Brain and Pituitary Transcriptome Analyses Reveal the Differential Regulation of Reproduction-Related LncRNAs and mRNAs in Cynoglossus semilaevis. Frontiers in Genetics, 12. ISSN 1664-8021

[thumbnail of pubmed-zip/versions/1/package-entries/fgene-12-802953/fgene-12-802953.pdf] Text
pubmed-zip/versions/1/package-entries/fgene-12-802953/fgene-12-802953.pdf - Published Version

Download (3MB)

Abstract

Long noncoding RNAs (lncRNAs) have been identified to be involved in half-smooth tongue sole (Cynoglossus semilaevis) reproduction. However, studies of their roles in reproduction have focused mainly on the ovary, and their expression patterns and potential roles in the brain and pituitary are unclear. Thus, to explore the mRNAs and lncRNAs that are closely associated with reproduction in the brain and pituitary, we collected tongue sole brain and pituitary tissues at three stages for RNA sequencing (RNA-seq), the 5,135 and 5,630 differentially expressed (DE) mRNAs and 378 and 532 DE lncRNAs were identified in the brain and pituitary, respectively. The RNA-seq results were verified by RT-qPCR. Moreover, enrichment analyses were performed to analyze the functions of DE mRNAs and lncRNAs. Interestingly, their involvement in pathways related to metabolism, signal transduction and endocrine signaling was revealed. LncRNA-target gene interaction networks were constructed based on antisense, cis and trans regulatory mechanisms. Moreover, we constructed competing endogenous RNA (ceRNA) networks. In summary, this study provides mRNA and lncRNA expression profiles in the brain and pituitary to understand the molecular mechanisms regulating tongue sole reproduction.

Item Type: Article
Subjects: STM One > Medical Science
Depositing User: Unnamed user with email support@stmone.org
Date Deposited: 06 Jan 2023 11:59
Last Modified: 02 May 2024 06:17
URI: http://publications.openuniversitystm.com/id/eprint/22

Actions (login required)

View Item
View Item